The news flash: Researchers at McMaster University claim to have discovered how massage treats muscle soreness after exercise1 (delayed-onset muscle soreness, or DOMS). This is a pretty hot news item, as allegedly good massage science news always is — everyone loves to love massage! — but there is too much hype and not enough good science. Unfortunately, there are several major problems with the study and its interpretation. The last time I checked, it’s just not safe to infer clinical mechanisms and benefits from a handful of biomarkers2 in a (very) small sample.
Inspired by the doubtful notion that “massage may relieve pain in injured muscle” after intense exercise3 researchers Crane et al looked for changes in the proteins that cells constantly make (“gene expression” — more on this below). They compared muscle tissue samples with and without massage and concluded that “massage therapy appears to be clinically beneficial by reducing inflammation and promoting mitochondrial biogenesis.” Mitochondria are the energy-producing organs of cells — cell power plants — so promoting their growth sounds super helpful. Contributor Simon Melov has been particularly enthusiastic in his pronouncements [video]:
Our research showed that massage dampened the expression of inflammatory cytokines in the muscle cells and promoted biogenesis of mitochondria.
And the journal Science Translational Medicine summarized the article like this:
Beneficial effects of massage on tired muscles work through anti-inflammatory and mitochondrial biogenesis pathways.
Sounds great, doesn’t it? If only we could believe it! The results of this study were actually negative: the data showed that massage has no significant effects on gene expression muscle in cells,4 and certainly none that clearly explain any therapeutic effect.
The research was interesting and worthwhile, but the hype is much too thick. The authors, the journal, the media, and massage therapists everywhere are all talking like it’s now a science fact (“It’s science!”) that massage “reduces inflammation” and “stimulates mitochondrial growth.” But really, this a flawed and limited study with an interpretation that is debatable at best, and it panders to what we’d like to believe about massage. It tries to explain an effect that doesn’t exist … an effect that massage didn’t really need. Massage feels great, regardless of whether it does anything to inflammation or mitochondria.
This is how myths are made, and it could well fall just as flat as the lactic acid myth — which is so ironic, because this paper actually put a nail in the coffin of that myth.5
The study was generally well-designed — the problems are almost entirely with interpretation. As massage studies go, it was clever, technical, and “science-y” and probably cost as much as a luxury sports car: Mark Tarnopolsky’s team profiled gene expression in the damaged muscles of eleven athletes, with and without massage therapy. That’s just cool, and newsworthy in itself.
So what is gene profiling? It’s a method of analyzing which genes are active — “expressed” — in a tissue sample. Genes are the code for proteins. So, this technique shows which proteins cells are busy cooking up.7 Specifically, they found a five-gene difference: cells in massaged muscle were, on average, making a little more or a little less of five proteins than cells in un-massaged muscle.
But how much more? How much less? Do we know what those proteins do? And how does this compare to changes in gene expression with exercise alone? I cannot emphasize enough how mind-bogglingly complicated this biology is.
In fact, the differences were quite small. “None of them were particularly strongly turned on or turned off.”8
What can we really make of a handful of small differences in gene expression? Truly, not much. The physiology is too complex to assume that these signals add up to anything therapeutic (or even benign).
Gene profiling is certainly interesting stuff and has great potential to solve many difficult problems in biology, but it is many steps removed from clinical reality and the actual results of a massage. The conclusions of the paper are no more than some educated, highly debatable guesses about the biological and clinical significance of those proteins … based on an older, simpler method of profiling to boot. Dr. Gorski:
These days, more sophisticated analyses, known as network analyses, are usually done. These involve looking for groups of genes that are turned on and off in synchrony that indicate broader pathways that are being turned on and off. Single genes don’t actually mean all that much. It’s the groups of genes going up and down together as part of a pathway that truly indicate specific pathways being turned on and off.
So it is one thing to know that a protein is being produced and quite another to know what happens to it after that, and what it means. The biological fate of any given protein depends on many factors. As with other high-tech diagnostic and research methods, gene profiling creates both a marvelous opportunity and the classic investigative hazard of “looking where the light is.”9 We tend to find and over-interpret whatever we can find and over-interpret. It’s extremely likely that other researchers could attempt the same research and get different results and/or come to different conclusions.
Clearly, exercise changes muscle cells — body builders are living proof of it. What about massage? Squishing muscle cells might cause them to behave differently, and it was worth using this method to check. Cells are always making proteins — it’s what they do, constantly — and if they change which proteins they are making after a massage, that could certainly be interesting science.
Crane et al found a difference all right, but size matters — effect size. Bear in mind that there are tens of thousands of genes.10 Five slight changes is no more than a biological blip.
It is all the more trivialized by a comparison to the effects of exercise. How much does a hard workout effect genetic activity? The effect of massage is simply dwarfed by the number of genetic changes — many hundreds, even thousands — caused by exercise alone. And what is the clinical significance of all those changes? Your muscles’ cells are clearly doing things dramatically differently after exercise, and yet all you feel is “sore and weak.” In other words, it takes a lot of cellular changes to add up to a relatively minor change in your experience.
It makes all kinds of biological and evolutionary sense that exercise changes muscle, and we know that it does, and that is reflected in the genetics. In contrast, there’s no particular reason to believe that massage would change cells much, and this data seems to clearly show that it doesn’t — certainly not compared to exercise.
It is unclear how applicable knowledge of infection-induced inflammation is to other types of inflammation.
— Ruslan Medzhitov, “Origin and physiological roles of inflammation”
A major premise of Crane et al’s research is that intense exercise damages muscle and that the soreness we experience for a day or two afterwards — delayed-onset muscle soreness, or DOMS — is caused by “inflammation,” and that’s bad, and we should stop it if we can. Inflammation is always treated like a villain, but it is much too complex to assume that it is villainous … or even present. Classic inflammation is mainly an infection-fighting system, and may have little or almost nothing in common with other recovery and healing processes.
The study identified only a few minor changes in gene expression, but inflammation itself generally involves many large changes in cellular behaviour. If massage were truly anti-inflammatory, it would cause much more significant changes — more like the changes caused by exercise itself.
One of the supposedly anti-inflammatory signs was a reduced production of cytokines (a gross oversimplification, but the same one that the authors of the paper chose). A cytokine is a class of proteins, and some of them are considered inflammatory. But cytokines are a large family of molecules used constantly by most cells for literally countless other reasons — so a small reduction in a particular cytokine is not smoking gun evidence of “reducing inflammation.”11 But even if it was, the decrease was truly unimpressive in size.
There’s an even more important, almost philosophical concern. DOMS isn’t particularly “inflammatory” to begin with — it’s radically different from classic inflammation, which is mainly an infection-fighting phenomenon. The immune system is not terribly worried about infection after a workout! The immune system is probably not terribly worried about infection after a workout. Anti-inflammatory medications like ibuprofen are considered anti-inflammatory because they suppress classic inflammation, and yet their effect on DOMS is distinctly underwhelming. In particular, their effect is notoriously “shallow”: they mask the pain without changing the duration or severity of DOMS, and you’re still just as weak.
There’s also evidence (and more pesky logic) that interfering with whatever is going on in DOMSy muscles could actually have negative consequences. The pain may be a side effect of a necessary and natural recovery process that you don’t really want to mess with much. “Reducing inflammation” in this context may not be a meaningful therapeutic goal, or one that is entirely safe or free of unintended consequences — for instance, we know that icing muscles may disrupt adaptation to exercise!12
Assuming there’s an anti-inflammatory effect at all.
This paper claims to have found an explanation for a therapeutic effect that doesn’t clearly exist, and this is where the gap between basic biology and clinical reality is most glaring: in the real world, massage does not reduce the pain of DOMS reliably or much.13 There is evidence that it helps, but the benefit is modest, “taking the edge off” at best. We can talk all day about cytokines and gene expression — whatever goes on in those cells, it does not add up to any significant pain reduction in people.
So we have here a classic case of an explanation in search of a phenomenon. And we’re a long way from a safe conclusion of clinical benefit. Perhaps the mitochondria thing?
“More mitochondria” is not actually what the paper found, although it sure does keep getting reported as if it did14 and is discussed in that spirit. Massage therapy bloggers everywhere seem to be celebrating more mitochondria. As with the inflammation, it’s much ado about nothing.
What the authors actually reported is: “potentiated mitochondrial biogenesis signaling” and “promotes mitchondrial biogenesis.” More specifically, they might have found “one molecule that promotes the development of mitochondria by 20% at most” (Gorski). They didn’t find more mitochondria … they found a single mitochondrial growth signal. It’s the difference between finding bigger plants or just a few bags of fertilizer. Potash in a farmer’s barn does not mean there is a bumper crop.
Whether or not mitochondrial growth actually happens depends on many biological inputs (like everything else that our cells do). Inferring from one signal in a small sample that “massage increases mitochondria” is an enormously optimistic leap. Here’s how another critic put it:
They literally haven’t shown that mitochondrial biogenesis is upregulated. Full stop. … There’s boatloads of assays these guys could have run in order to probe for higher levels of mitochondrial biogenesis, not to mention a plethora of genes related to biogenesis, fission, and fusion whose mRNA levels were not analyzed. Weak sauce.
— ScienceBasedMedicine.org commenter TheLabMix (“a researcher who works on mitochondria”)
The mitochondria claim here doesn’t make much biological sense,15 but it doesn’t explain anything about massage. There are no known effects of massage that have anything to do with growing more mitochondria. No such effect is even suspected or unwisely claimed. Since mitochondria provide energy to cells, growing more of them would improve muscle performance — a big deal. Imagine if that was true! Massaged athletes would have impressive, measurable advantages … which we already know is simply not the case. Massage is great stuff, but it’s not the secret sauce of Olympic champions.
Simon Melov said of his research: “There's general agreement that massage feels good, now we have a scientific basis for the experience.” We don’t have any such thing: it’s a few scraps of basic biology with possible but unproven and unlikely relevance to massage effects that are minor at best. That’s half-baked. Over-interpretation of data like this is why “most research is wrong” — the more complex and further removed biology is from clinical outcomes, the more ways there are for it to get misinterpreted and distorted even by the researchers themselves (never mind science journalists and clinicians).16
More importantly, we already have a scientific basis for why massage feels good which is perfectly understandable17 without resorting to a half-baked claim that massage treats inflammation, or even fully baked proof of it. Most people do not generally get massage to cure inflammation or DOMS! Post-event massage after intense exercise is fairly common among elite athletes, and doubtless that was the inspiration for the research, but such extreme exertions are irrelevant to most athletes,18 Certainly this research has nothing to do why the average person buys massage.19 Even if the paper is correct in every way — perhaps massage really is meaningfully anti-inflammatory — that would not remotely explain how most massage works.
The proper conclusion of this study is that there was little or no significant effect in … gene expression from massage. That would have been perfectly fine as a conclusion. After all, negative studies happen and should be published. Yet that’s not what was concluded. They did not report what was in essence a negative study.Dr. Gorski’s analysis is thorough and much more technical than mine — he has done gene-profiling research — but well worth at least scanning through. Agree or disagree, his criticisms are so numerous and serious that it’s clear that the study can’t be a slam-dunk. BACK TO TEXT
It’s only one study, but … yikes! This fascinating experiment done by Japanese researchers showed that regular icing for a few weeks after workouts resulted in a significant reduction in training effects: ice users didn’t get as strong. This implies that icing may interfere with normal post-exercise muscle physiology and prevent the process of muscles adapting to stress. This is reinforced by Tseng et al.BACK TO TEXT
This intensely intellectual paper — it’s completely, hopelessly nerdy — became one of the most downloaded articles in the history of the Public Library of Science and was described by the Boston Globe as an instant cult classic. Despite the title, the paper does not, in fact, say that “science is wrong,” but something much less sinister: that it should take rather a lot of good quality and convergent scientific evidence before we can be reasonably sure of something, and he presents good evidence that a lot of so-called conclusions are premature, not as “ready for prime time” as we would hope. This is not the least bit surprising to good scientists, who never claimed in the first place that their results are infallible or that their conclusions are “true.”BACK TO TEXT